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Size-exclusion chromatography

Size-exclusion chromatography, also known as steric-exclusion chromatography, is used to separate compounds according to their molecular size from exclusion gel particles.

  • Small molecules will penetrate into the pores of the gel particles, and will be strongly retained inside the column.
  • Large molecules will not be able to penetrate the pores at all and will therefore be excluded: they will migrate quickly with the mobile phase.
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