UV spectrophotometry analysis

EXAMPLE OF DETERMINATION

First example, paracetamol:
  • Raw material
  • Lindilane®

Second example, sulfamethoxazole:
  • Raw material
  • Bactrim®

C10H11N3O3S  ;   Mr : 253.3 g.mol-1
 
Sulfamethoxazole in the form of raw material or in the form of a Bactrim® tablet (combined with trimethoprim) will be quantified using UV spectrophotometry.

Acidic medium: lmax : 265nm ; E1% = A11 = 175
Basic medium: lmax : 256nm ; E1% = A11 = 673 
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Equipment and reagents:
  • graduated flasks
  • wash bottle
  • propipette
  • pipettes
  • solvent
  • mortar
  • weighing cup
  • spatula
  • spectrophotometer
  • UV cuvette
This method requires:
  • the appropriate equipment
  • suitable personal and collective protection (lab coat, gloves, glasses, fume hood).
  • Estimated duration: 1-2 h.
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