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How to perform a TLC?
Introduction
A little theory
Experimental procedure
Illustrating theory
Get some practice
To find out more
Quiz: What can you remember about TLC?
What are the benefits of chamber saturation?
Allows us to obtain Rf higher than 1
Maintains a sufficient volume of eluent
Ensures reproducible migration
Allows for the colored development of eluted products
Indicate whether the characteristics below are suitable for a product deposited on TLC to be visible directly under a UV lamp
The compound can absorb in infrared
The compound must be colored
The compound can be colored
The compound must be volatile
The compound must have absorbent groups under UV irradiation
Indicate whether the solvents or mixtures below can constitute eluents
Water / cyclohexane
Diethyl ether / petroleum ether
Dimethylformamide
Dichloromethane / ethanol
Ethyl acetate
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