Basics of HPLC

Exercise 2: Reversed phase chromatography

I) Rank the solvents in ascending order of eluting power for compounds retained on a C18-bonded silica column.

II) Still using a C18-bonded silica column, we wish to perform a gradient elution between two solvents, one of which is water. Which solvent(s) cannot be used to make this gradient?

Dichloromethane
Methanol
Acetone
Ethyl acetate
Hexane
Acetonitrile
Ether

Correction

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