Carrying out a calibration involves a succession of steps:
1- Choose the range of concentrations to cover for the compound to be dosed, and the calibration points of the range.
2- Calculate the dilutions and volumes to sample in order to have these calibration points available, knowing the concentration of the stock solution of the compound to be dosed (and making sure that there is a sufficient volume of stock solution to carry out the calibration).
3- Take samples of the stock solution and make dilutions, to obtain the range of different calibration points; stir the different standard solutions prepared in this way.
4- Take an aliquot of each solution in order to analyze the different calibration points with the measuring device (e.g. UV-spectrophotometer, after having placed an aliquot of each standard solution in a suitable cuvette).
5- Plot the measurement results for each point in the range to draw the calibration curve.
6- Analyze unknown samples with the same measuring instrument (and on the same day), so as to quantify the compound to be dosed in these samples using the calibration curve.
Stay within the linear range of the instrument
Write down the calculations to avoid making mistakes!!