How to perform a TLC?

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Experiment A: influence of volatility

Collect the liquids below in vials:

  1. toluene
  2. acetone
  3. ethanol
  4. dimethylformamide
  5. dichloromethane

Using capillaries, make the deposits of the previous liquids (by separating each deposit by about 1 cm). Observe IMMEDIATELY under a UV lamp, then again a few minutes later. Conclude.


2 inserts: visualize the TLC results and Conclusion on this experiment.

Observation under UV lamp of 3 solvent deposits:
Toluene – Ethanol - Acetone
a) Right after the deposit
b) 1 min after the deposit
We observe that:
  • toluene and acetone are volatile since they are no longer revealed a minute after the deposit
  • ethanol does not absorb under UV.
Click on each box to see the result
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