What is the aim of the experiment?
To establish the existing relationship between the measurement result and the quantity (or concentration).


Several types of calibration according to the measurement method: external, internal, standard additions. This sheet will present external calibration in further detail.
What is its purpose?
To estimate the amount (or concentration) of a target compound (molecule/ion/atom) in a sample.
Illustration of a graph, with an abscissa "Measurement" and an ordinate "Quantity or concentration". It shows an increasing and constant straight line, having for origin the point 0.
Practical constraints
Having a standard available (target compound in the case of external calibration or standard additions, similar compound if internal calibration).
Having access to the measuring device (spectrophotometer, HPLC-UV, or other depending on the intended analysis).
Stay within the linear range of the instrument, if possible.
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