How to perform a TLC?

General instructions

It is proposed here to study the different parameters that can affect the quality of a TLC and the visualization of the deposited compounds.
Some organic products will be used here as a support to highlight these parameters.

Before performing a TLC, you must:

  1. Index vials and pour the different solutions into these containers
  2. Write the number of the experiment on the various TLCs boldly in pencil
  3. Remember to firmly close the vials containing solvents or solutions.
  4. Carry out the deposits, the introduction of the TLC into the chamber, as well as the elutions of the solutions under a hood.
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