Basics of GC

The injector’s role


  • To introduce into the chromatograph a precise volume of sample for analysis without disturbing the flow of the gaseous mobile phase in the system.
  • For non-volatile samples at room temperature, to vaporize them so that they are transported towards the separation column by the carrier gas.

The injector’s role


  • To vaporize the sample.
  • To introduce the volatile compounds into the separation column.

The injector’s role


  • To prevent the distortion of chromatographic peaks by rapid injection and non-saturation of the stationary phase: division mode (split).

The injector’s role


  • Very diluted sample in a solvent, no risk of column overloading: without division (splitless mode)
  • Splitless mode in two stages:
    • Transfer of the sample to the column head with condensation of solvent
    • Solvent vaporization and initiation of analyte elution

Split/splitless injection?


Adapting the injection type to the sample, especially taking into account its concentration.
Transcript : French English
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