Before analysis of the standard solutions, a blank determination is recommended (e.g. in spectrophotometry, distilled water will be used to set zero). The standard solutions must then be run in random order.
Interactive diagram: virtual analysis of the standard solutions
of the calibration with a spectrophotometer
of the calibration with a spectrophotometer
In case of possible memory effects between solutions, running the solutions in an ascending order of concentration would not reveal such effects – this is why a random running order is recommended.