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Determination of NO2- ions using spectrophotometry

Dosing principle

In the presence of dichloride of N-(naphtyl-1) diamino-1,2 ethane at pH 1.9 there is a diazotization reaction of amino-4 benzenesulfonamide by the nitrites. The pink complex created is measured spectrophotometrically at a wavelength close to 540 nm.

A calibration line is used to relate the absorbance to the nitrite concentration.

Refer to the calibration sheet

Illustration of a standard range for the determination of nitrite ions. 6 vials contain a violet liquid of increasingly pronounced color.
Warning pictogram
Precautions for use: Filtering the water before dosing is preferable since the presence of suspended matter is capable of falsifying the colorimetric measurement.
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